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Interactions between enzymes and oxide colloidal particles and their influence on enzymatic activity

Ludmilla Derr

Reihe: Grund- und Werkstoffe/Kunststoffe

Band 760 2016

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Enzymatic activity S. 41-47

„… 6 Enzymatic activity ABSTRACT: Chapter 6 provides a detailed introduction to Michaelis-Menten …” „… Michaelis-Menten constant KM and substrate efficiency. A central assay for the analysis of the enzymatic activity …” „… Hanes [ S ] [S]/v -Km KM/Vmax 1/Vmax Eadie- Hofstee v / [ S ] V Vmax/Km Vmax -Km 6.3 Activity assays …”

Effects of silica and alumina colloidal particles on enzymatic activity of achymotrypsin after adsorption S. 82-102

„… 9 Effects of silica and alumina colloidal particles on the enzymatic activity of achymotrypsin …” „… substrate was optimized to enable the assessment of enzymatic activity for adsorbed Chymotrypsin. The …” „… , 179, 181‘183. a large number of studies have investigated enzymatic activity before and after …”

Conclusions S. 164-167

„… , physisorption was seiected to examine the effects of colloidal ceramic particles on the enzymatic activity of …” „… colloidal particles, adsorption negatively influenced the enzymatic activity of adsorbed Chymotrypsin, as …” „… indicated by substantial losses in enzymatic activity. Approximately 12-15 %, 7-9 %, and 6-9 % of the …”

Titelei/Inhaltsverzeichnis S. I-XX

„… and their influence on enzymatic activity v d i v e r l a g Derr, Ludmilla Interactions between …” „… enzymes and oxide colloidal particles and their influence on enzymatic activity Fortschr.-Ber. VDI Reihe …” „… protein-particle adsorption 39 6 Enzymatic activity 41 6.1 Michaelis-Menten kinetics 41 6.2 Determination of KM and …”

Physisorption of enzymatically active chymotrypsin on titania colloidal particles S. 59-81

„… , we observe that the adsorption is accompanied by a substantial loss of enzymatic activity, and only …” „… reagents with the activity assay36' 157. A well-known enzymatic assay based on the hydrolysis of the …” „… enzymatic activity is retained after binding on the particle surface. 60 8.2 Experimental section 8.2.1 …”

Assessment of proteolytic activity of a-chymotrypsin immobilized on colloidal particles by matrix-assisted laser desorption ionization time-of-flight mass spectroscopy S. 140-163

„… enzymatic activity of the bound Chymotrypsin at different times, in consecutive proteolytic cycles and …” „… of enzyme activity and overall enzymatic performance at different operation and storage conditions …” „… 258'261. In this study we investigate at a semi-quantitative level the enzymatic catalytic activity of …”

Introduction S. 1-4

„… structure, thoroughly investigated enzymatic activity and a wide ränge of possible applications. Two key …” „… to obtain because enzymatic activity may be altered by various factors, including orientation on …” „… this optimized assay, the enzymatic activity of covalently immobilized Chymotrypsin was estimated by …”

Outlook S. 168-169

„… the catalytic activity of the adsorbed enzymes. Second, it is also feasible to investigate the …” „… influence of surface functionalization of the ceramic colloidal particles on the enzymatic activities of …” „… easier to accomplish because the methods and the optimized enzymatic assay described in this study can …”

Experimental methods and principles S. 48-58

„… Activity assessment of free and adsorbed achymotrypsin The enzymatic activity of free Chymotrypsin in …” „… the enzymatic activity of adsorbed enzymes is that assays based on UV/Vis spectrometry are not …” „… extinction signals in a linear measurement ränge. Consequently, the enzymatic activity of adsorbed …”

References S. 173-194

„… ., Adsorption-induced changes in ribonuclease A structure and enzymatic activity on solid surfaces. Langmuir 2014,30 …” „… nanoparticle size influences the structure and enzymatic activity of adsorbed lysozyme. Langmuir 2004,20, (16 …” „… , R., Improvement of enzyme activity, stability and selectivity via immobilization techniques. Enzyme …”

Physisorption of a-chymotrypsin on SiO2 and TiO3: a comparative study via experiments and molecular dynamics simulations S. 103-139

„… according to manufacturer's instructions. The enzymatic activity of Chymotrypsin was determined according …” „… incubation time of 20 h, the Chymotrypsin concentration and the enzymatic activity in the supernatant were …” „… prolonged incubation and its enzymatic activity are determined and reported in Figure 10.5. Immediately …”

Protein adsorption S. 34-40

„… changes can have a strong effect on protein function, with positive or negative changes in enzymatic …” „… activity13' 22, 95, 96. Furthermore, lateral interactions between neighboring adsorbed proteins can have …”

doi.org/10.51202/9783185760051

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Gefahrstoffe

Alle Ausgaben: Gefahrstoffe

GrdL Jahrgang 81 (2021) Heft 11-12 ISSN Online: 0949-8036

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Jäckel. Udo, Inken Laufer, Holme Kühne, Nancy Heubach, Nicole Hüttig Endotoxin measurements in the PM10 fraction of ambient air/Endotoxinmessungen in der PM10-Fraktion der Außenluft S. 443-446

„… A part, which is responsible for their endotoxic activity [1]. Endotoxins are con tinuously …” „… biological test which is based on the activation of factor C of the enzymatic coagulation A B S T R A C T …” „… monomers instead of endotoxin aggregates leads to lower activity in LAL assay [37]. In previous studies …”

doi.org/10.37544/0949-8036-2021-11-12

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37. Internationales Wiener Motorensymposium 28. – 29. April 2016

Hans Peter Lenz (Hrsg.)

Reihe: Verkehrstechnik/Fahrzeugtechnik

Band 799 2016

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R. Schlögl Neue Kraftstoffe aus CO2/CO2 to Fuels – Chemical Perspectives S. II-253-II-264

„… enzymatic processes. We must always avoid the formation of carbonates as this leads …” „… by enzymatic processes using sugar as starting compound (fermentation). The reader can …” „… - Pedersen, F.;; Temel, B.;; Schjodt, N. C.;; Norskov, J. K.;; Studt, F., Activity and …”

doi.org/10.51202/9783186799128

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Near-net-shape fabrication of open-porous bone replacement materials of calcium phosphate and protein

Berit Müller

Reihe: Biotechnik/Medizintechnik

Band 294 2017

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DISCUSSION S. 64-75

„… impair enzymatic activity [144, 145]. However the extent of damage by gamma-sterilisation may vary …” „… scaffold. An impact of gamma-sterilisation on LSZ activity could not be discovered. This goes along with …”

doi.org/10.51202/9783186294173

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Model-Based Design of Pure and Multicomponent Cellulosic Biofuels for Advanced Engine Concepts

Manuel Dahmen

Reihe: Verfahrenstechnik

Band 954 2017

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Bibliography S. 227-276

„… squares (PLS). Quantitative Structure-Activity Relationships, 12(2):137–145. Climent, M. J., Corma, A …” „… . Quantitative Structure-Activity Relationships, 7(1):18–25. Creton, B., Dartiguelongue, C., de Bruin, T., and …” „… ). How not to develop a quantitative structure–activity or structure–property relationship (QSAR/QSPR …”

Basic concepts of fuel design S. 10-39

„… monosaccharides such as glucose (C6H12O6) and xylose (C5H10O5) by enzymatic or acid hydrolysis (Serrano-Ruiz and …” „… ., 1975), i.e., a GCM frequently used for the prediction of liquid-phase activity coefficients. However …” „… a mathematical relationship between the property (or the activity) of interest and a set of …”

doi.org/10.51202/9783186954039

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Reaction Network Generation and Evaluation for the Design of Biofuel Value Chains

Juan José Wilhelm Victoria Villeda

Reihe: Verfahrenstechnik

Band 950 2017

doi.org/10.51202/9783186950031

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