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Interactions between enzymes and oxide colloidal particles and their influence on enzymatic activity

Ludmilla Derr

Series: Grund- und Werkstoffe/Kunststoffe

Volume 760 2016

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Introduction S. 1-4

„… research is enzyme immobilization, which offers advantages such as increased stability, higher catalytic …” „… extremely expensive and time-consuming, enzyme immobilization offers a welcome simplification of the …” „… Separation and recovery processes7,11"18. Various enzyme immobilization strategies have been employed for …”

Assessment of proteolytic activity of a-chymotrypsin immobilized on colloidal particles by matrix-assisted laser desorption ionization time-of-flight mass spectroscopy S. 140-163

„… , lack of long-term stability and cyclic reusability, enzyme immobilization onto solid supports is …” „… considered a promising 140 strategy to overcome these main drawbacks17. Immobilization of the enzyme onto …” „… these aspects have made enzyme immobilization particularly attractive for downstream processes or …”

Conclusions S. 164-167

„… adsorbed or covalently immobilized enzymes. Because the interplay of interactions between enzymes and …” „… applications. Two main immobilization strategies, physisorption and covalent immobilization, were employed in …” „… structures were identified. The following trends were determined for enzyme adsorption in all chosen …”

Titelei/Inhaltsverzeichnis S. I-XX

„… surface functionalization and covalent enzyme immobilization 27 4.1 Alumina colloidal particles 27 4.2 …” „… of colloidal particles 30 4.5 Covalent enzyme immobilization 31 5 Protein adsorption 34 5.1 …” „… and difficult to predict. Enzyme immobilization on inorganic oxide particles is a widely employed …”

References S. 173-194

„… ., Chapter 7 - Nanostructured materials for enzyme immobilization and biosensors. In The New Frontiers of …” „… ), 7- 25. 5. Monzo, A.; Sperling, E.; Guttman, A., Proteolytic enzyme-immobilization techniques for …” „… , R., Improvement of enzyme activity, stability and selectivity via immobilization techniques. Enzyme …”

Colloidal particles, their surface functionalization and covalent enzyme mobilization S. 27-33

„… 4 Colloidal particles, their surface functionalization and covalent enzyme immobilization …” „… . Selected methods o f covalent enzyme immobilization w ill be explained in detail. a- A lu m in a A m o rp …” „… covalent enzyme immobilization80'82. CMj Hydrolysis •jMJ NH, tCHj), (CH,), 5: OH + HO----Si----OH 1 Oh OH …”

Outlook S. 168-169

„… immobilization methods applied in this study, physisorption and covalent immobilization, should be considered …” „… separately. The study of adsorption interactions between enzymes and ceramic colloidal particles can be …” „… extended in two ways. First, enzyme engineering methods could be applied to modify enzyme properties such …”

List of students projects S. 170

„… "Enzyme immobilization", Student Assistant, 2012 Cagla Cengel "Modellbildung zur Ladungsverteilung auf …” „… Proteinoberflächen", Semester Project, 2011 Sonia Jahn "Enzyme immobilization", Student Assistant, 2012-2013 …” „… Supreet Kaur "Enzyme adsorption", Student Assistant, 2013 Ertugrul Kuzu „Untersuchungen der …”

Effects of silica and alumina colloidal particles on enzymatic activity of achymotrypsin after adsorption S. 82-102

„… insoluble support materials for enzyme immobilization7' 9' 13, 19, 178‘ 180. Due to their numerous …” „… practical applications, proteolytic enzymes are also often employed for immobilization studies146, 152 …” „… immobilization14'17. Studies using different analysis methods to assess the activity of immobilized enzymes are …”

Physisorption of enzymatically active chymotrypsin on titania colloidal particles S. 59-81

„… Introduction Immobilization of enzymes onto water-insoluble carriers is of increasing importance for a wide …” „… , pharmaceutics and proteomics6'8' 140. Enzyme immobilization offers several advantages including more …” „… reuse of costly enzymes. In the past decades, different immobilization approaches have been designed …”

Enzymatic activity S. 41-47

„… before and after immobilization on colloidal particles require a highly reproducible enzyme assay. A …” „… introduced. 6.1 Michaelis-Menten kinetics A simple enzyme-catalyzed reaction with one substrate is analogous …” „… an enzyme-catalyzed reaction. The curve shown in Figure 6.1 can be obtained by calculating the …”

Physisorption of a-chymotrypsin on SiO2 and TiO3: a comparative study via experiments and molecular dynamics simulations S. 103-139

„… insoluble supports15,17,18. Immobilization confers enzymes higher stability towards temperature variations …” „… easier Separation from other dissolved compounds3"6, 140. Immobilization 103 of enzymes on solid supports …” „… between immobilized enzymes and ceramic supports, we compare the adsorption features of Chymotrypsin on …”

doi.org/10.51202/9783185760051

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Near-net-shape fabrication of open-porous bone replacement materials of calcium phosphate and protein

Berit Müller

Series: Biotechnik/Medizintechnik

Volume 294 2017

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REFERENCES S. 81-92

„… the immobilization of proteins. Materialwissenschaft Und Werkstofftechnik, 2005. 36(12): p. 820- 827 …” „… . 427-436. 26. Carturan, G., et al., Inorganic gels for immobilization of biocatalysts: inclusion of …” „… . 173-315. 48. Yang, V.C., J.F. Liang, and Y.T. Li, Biomedical application of immobilized enzymes. Journal …”

doi.org/10.51202/9783186294173

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Model-Based Design of Pure and Multicomponent Cellulosic Biofuels for Advanced Engine Concepts

Manuel Dahmen

Series: Verfahrenstechnik

Volume 954 2017

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Basic concepts of fuel design S. 10-39

„… of enzymes and strains has yielded sufficiently fast production pathways, strategies like cell …” „… immobilization, retention or recycling and in-situ product recovery can be employed to optimize a bio-catalytic …”

doi.org/10.51202/9783186954039

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