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Interactions between enzymes and oxide colloidal particles and their influence on enzymatic activity

Ludmilla Derr

Series: Grund- und Werkstoffe/Kunststoffe

Volume 760 2016

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Assessment of proteolytic activity of a-chymotrypsin immobilized on colloidal particles by matrix-assisted laser desorption ionization time-of-flight mass spectroscopy S. 140-163

„… lysozyme-derived peptides with MALDI-ToF-MS (Figure 11.2). When lysozyme was exposed to either Chymotrypsin …” „… demonstrated by the MALDI-ToF-MS observation of multiple lysozyme-derived peptides after proteolytic …” „… lysozyme-derived peptides is clearly induced by the presence of Chymotrypsin on the particles' surface as no …”

Conclusions S. 164-167

„… of lysozyme-derived peptides was obviously induced by the presence of Chymotrypsin on the particle …” „… Chymotrypsin was estimated by MALDI-ToF-MS using lysozyme as the enzyme substrate and analyzing multiple …” „… , roughly the sam e num ber of lysozyme peptide sequences could be identified, indicating active lysozyme …”

Titelei/Inhaltsverzeichnis S. I-XX

„… (MALDI-ToF-MS) using lysozyme as the enzyme substrate to analyze multiple lysozyme-derived peptides after …” „… surface density of the adsorbed enzymes. Covalent im mobilization: • The formation of lysozym e-derived …” „… reaction and possible steric hindrance. • Both materials retained lysozyme digestion activity after 7 weeks …”

List of publications S. 171

„… . Adsorption orientation and binding motifs of lysozyme and Chymotrypsin on amorphous silica. J. Phys. Chem. C …”

Outlook S. 168-169

„… Chymotrypsin in the performed reusability study indicated that the immobilized Chymotrypsin retained lysozym e …”

Physisorption of enzymatically active chymotrypsin on titania colloidal particles S. 59-81

„… activity of adsorbed lysozyme on fumed silica at different conditions. A 60% loss of activity was found …” „… when lysozyme formed approximately a monolayer. Reduction of enzymatic activity is considered to be …” „… adsorbed lysozyme as suggested by structural studies. Concerning potential technical applications of …”

References S. 173-194

„… ), 4585-4602. 30. Etheve, J.; Dejardin, P., Adsorption kinetics of lysozyme on silica at pH 7.4: Correlation …” „… .; Gauckler, L. J., Lysozyme and bovine serum albumin adsorption on uncoated silica and AlOOH-coated silica …” „… nanoparticle size influences the structure and enzymatic activity of adsorbed lysozyme. Langmuir 2004,20, (16 …”

Introduction S. 1-4

„… matrix-assisted laser desorption/ ionization time-of-flight mass spectroscopy (MALDI-ToF-MS) using lysozyme as an …”

Experimental methods and principles S. 48-58

„… a-Chymotrypsin 10 34 0 20 36 Trypsin 8 32 0 30 30 Lysozyme 36 9 0 32 23 Hemoglobin 75 0 0 14 11 7.7 Visual …”

doi.org/10.51202/9783185760051

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Near-net-shape fabrication of open-porous bone replacement materials of calcium phosphate and protein

Berit Müller

Series: Biotechnik/Medizintechnik

Volume 294 2017

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Titelei/Inhaltsverzeichnis S. I-XIV

„… – Bioresorbability – Bone Replacement – Calcium Phosphate – Freeze Gelation – Local Drug Release – Lysozyme – Serum …” „… ) ......................................................................................... 13 2.5.2 Lysozyme (LSZ …” „… . Two at neutral pH oppositely charged model proteins, bovine serum albumin with an acidic and lysozyme …”

REFERENCES S. 81-92

„… egg-white lysozyme. J Mol Biol, 1974. 82(3): p. 371-91. 36. Rezwan, K., et al., Change of xi potential of …” „… biocompatible colloidal oxide particles upon adsorption of bovine serum albumin and lysozyme. Journal of …” „… prediction method for the isoelectric point of binary protein mixtures of bovine serum albumin and lysozyme …”

AUTHOR’S PUBLICATIONS S. 93-96

„… Lysozyme Adsorption on Calcium Phosphate Particles Advanced Engineering Materials, 2010 (Vol. 12, I. 1-2 …” „… ) Mueller, B.; Treccani, L.; Rezwan, K. Antibacterial active open-porous hydroxyapatite/lysozyme scaffolds …”

INTRODUCTION S. 1-3

„… serum albumin (BSA) and lysozyme (LSZ) were chosen. The structure of both proteins is well known …” „… delivery matrices. For this purpose hydroxyapatite/lysozyme scaffolds were fabricated. Lysozyme is an …”

BASICS S. 4-14

„… lysozyme [47]. Methods for administering drugs can be divided into two categories: systemic and local …” „… (BSA) and lysozyme (LSZ), were chosen to be useful for a variety of applications. 2.5.1 Bovine serum …” „… Lysozyme (LSZ) LSZ is an enzyme with antibacterial effect. For protecting the human body of infection it …”

RESULTS S. 39-63

„… Serum Albumin and Lysozyme Adsorption on Calcium Phosphate Particles. Advanced Engineering Materials …” „… hydroxyapatite/lysozyme scaffolds suitable as bone graft and depot for localised drug delivery. Journal of Biomaterials …” „… without protein (w. p.), with 5 wt.% chicken egg white lysozyme (LSZ), and 5 wt.% porcine serum albumin …”

EXPERIMENTAL S. 27-38

„… hrs. The proteins, albumin from bovine serum (A7906, lot no. 036K0722) and lysozyme from chicken …” „… , purity > 98 %), porcine serum albumin (Cat No A1830, purity > 98%), lysozyme from chicken egg white (Cat …”

DISCUSSION S. 64-75

doi.org/10.51202/9783186294173

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Richtig handeln bei Wasser- und Feuchtigkeitsschäden.

Michael Grübel

2021

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3 Schäden an Wänden oder Zwischendecken S. 54-155

doi.org/10.51202/9783738806144

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